The　present　manuscript　intended　to　investigate　the　anti-inflammatory　effect　of　ropivacaine　on　lipopolysaccharide-induced　inflammation　in　RAW　264.7　macrophages.　Results　suggested　that　ropivacaine　causes　significant　inhibition　of　generation　of　nitric　oxide　(NO),　prostaglandin　E2,　tumor　necrosis　factor　(TNF)-α,　interleukin　(IL)-6,　and　IL-1β,　as　well　as　expression　of　their　synthesizing　enzymes,　inducible　NO　synthase,　and　cyclooxygenase-2.　Moreover,　ropivacaine　causes　inhibition　of　mitogen-activated　protein　kinases　as　well　as　nuclear　factor-kappa　B　signaling　pathway　and　apoptosis　in　RAW　264.7　cells.　Based　on　the　results,　it　has　been　suggested　that　ropivacaine　showed　beneficial　effect　against　inflammation.