Multidrug-resistant　bacterial　(MDRB)　infections　are　still　a　critical　challenge　in　tissue　engineering　and　regenerative　medicine.　Bioactive　biodegradable　scaffolds　with　the　ability　to　combat　MDRB　infections　and　that　have　high　biocompatibility　could　likely　address　this　problem.　Herein,　we　develop　a　biomimetic　photoluminescent　and　antibacterial　gelatin-poly(citrate-siloxane)-epsilon-poly(L-lysine)　(GT-PCS-EPL)　nanofibrous　scaffold　for　multifunctional　tissue　regeneration　applications.　GT-PCS-EPL　had　biomimetic　elastomeric　behavior;　tunable　photoluminescence;　broad-spectrum　antibacterial　activity,　including　against　MDRB　infections;　and　enhanced　osteogenic　bioactivity　capacity.　The　photoluminescent　property　of　GT-PCS-EPL　scaffolds　enabled　their　real-time　noninvasive　imaging　tracking　in　vivo.　GT-PCS-EPL　scaffolds　not　only　killed　normal　bacteria　but　also　efficiently　treated　MDRB　growth　in　vitro　and　infections　in　vivo.　GT-PCS-EPL　scaffolds　also　had　excellent　hemocompatibility　and　osteoblast　biocompatibility.　This　study　provides　a　novel　strategy　to　design　biomimetic　bioactive　scaffolds　with　multifunctional　properties　for　smart　infection-related　tissue　regeneration　applications.