Further　to　conventional　linear,　branched,　crosslinked,　and　dendritic　polymers,　single　chain　cyclized/knotted　polymers　(SCKPs)　have　emerged　as　a　new　class　of　polymer　structure　with　unique　properties.　Herein,　the　development　of　bacteria-resistant　SCKPs　is　reported　and　the　effect　of　this　structure　on　the　resistance　of　polymer　materials　to　bacteria　is　investigated.　Four　SCKPs　were　synthesized　by　reversible　addition　fragmentation　chain　transfer　(RAFT)　homopolymerization　of　multivinyl　monomers　(MVMs)　and　then　crosslinked　by　UV　light　to　form　SCKP　films.　Regardless　of　MVM　type　used,　the　resulting　SCKP　films　showed　much　higher　resistance　to　bacteria,　and　up　to　75　%　less　bacterial　attachment　and　biofilm　formation,　in　comparison　with　the　corresponding　non-SCKP　films.　This　is　due　to　the　altered　surface　morphology　and　hydrophobicity　of　the　SCKP　films.　These　results　highlight　the　critical　role　of　the　SCKP　structure　in　enhancing　the　resistance　of　polymeric　materials　to　bacteria.