The　Charpy　impact　absorbed　energy　of　a　hot-rolled　Ti–6Al–3Nb–2Zr–1Mo　alloy　plate　with　different　sampling　and　notching　directions　was　investigated.　Strong　anisotropic　variations　occurred　in　impact　absorption　energies　due　to　difference　of　the　α　phase　crystal　orientation　and　microstructural　characteristics　along　different　directions.　The　higher　crack　propagation　energy　of　the　sampling-notching　direction　sample　(RD-ND)　resulted　in　an　impact　absorption　energy　of　approximately　2.5　times　larger　than　that　of　the　TD-ND,　TD-RD　and　RD-TD　samples.　Based　on　the　analysis　of　the　α　crystal　orientation　and　the　TEM　microstructure　after　impact　deformation,　it　was　found　that　the　higher　absorption　energy　of　the　RD-ND　sample　was　attributed　to　the　activities　of　a　large　number　of　{101‾0}12‾10　prismatic　slips　and　the　resistance　created　by　grain　boundaries　against　crack　propagation,　which　also　resulted　in　the　delamination　of　the　impact　fracture　surface.　Finally,　the　coupling　effect　of　crystallographic　texture　and　microstructural　characteristics　on　the　impact　deformation　mechanisms　of　the　hot-rolled　Ti–6Al–3Nb–2Zr–1Mo　alloy　was　described.　©　2021　Elsevier　B.V.