To　investigate　the　transdifferentiation　of　bone　marrow-derived　mesenchymal　stem　cells　(BMSCs)　into　salivary　gland-like　cells　via　a　novel　culture　method　employing　induction　culture　medium　collected　from　salivary　gland　cells.　Primary　salivary　gland　cells　were　cultured,　and　after　the　first　passage,　the　culture　medium　was　collected　for　use　as　induction　medium.　BMSCs　(passage　3)　were　cultured　in　either　induction　medium　(induction　group)　or　DMEM/F12　medium　with　10　%　(v/v)　fetal　bovine　serum　(control　group)　before　seeding　on　three-dimensional　collagen/chitosan　scaffolds　and　subcutaneous　transplantation　into　nude　mice.　The　in　vitro　and　in　vivo　transdifferentiation　of　BMSCs　into　salivary　gland-like　cells　was　evaluated　by　immunocytochemical　analysis　of　alpha-amylase　and　cytokeratin-8　(CK-8)　expression.　Salivary　gland-like　cells　cultured　using　this　novel　method　maintained　excellent　biostability　and　exhibited　relatively　stable　expression　of　alpha-amylase　and　CK-8　in　vitro　and　in　vivo.　This　novel　culture　method　is　feasible　for　inducing　the　transdifferentiation　of　BMSCs　into　salivary　gland-like　cells.