A　growing　number　of　studies　have　indicated　that　microRNAs　(miRNAs)　are　critical　regulators　of　carcinogenesis　and　cancer　progression　and　may　serve　as　potential　therapeutic　tools　for　cancer　therapy.　Frizzled7　(Fzd7),　the　most　important　receptor　of　the　Wnt　signaling　pathway,　is　extensively　involved　in　cancer　development　and　progression.　However,　the　role　of　Fzd7　in　prostate　cancer　remains　unclear.　In　this　study,　we　aimed　to　explore　the　expression　of　Fzd7　in　prostate　cancer　and　test　whether　modulating　Fzd7　expression　by　miR-613　would　have　an　impact　on　prostate　cancer　cell　proliferation　and　invasion.　We　found　that　Fzd7　was　highly　expressed　in　prostate　cancer　cell　lines.　Through　bioinformatics　analysis,　Fzd7　was　predicted　as　a　target　gene　of　miR-613,　which　was　validated　by　dual-luciferase　reporter　assays,　realtime　quantitative　polymerase　chain　reaction　and　Western　blot　analysis.　By　gain　of　function　experiments,　we　showed　that　overexpression　of　miR-613　significantly　suppressed　prostate　cancer　cell　proliferation　and　invasion.　Furthermore,　miR-613　overexpression　markedly　downregulated　the　Wnt　signaling　pathway.　Through　a　rescue　experiment,　we　showed　that　overexpression　of　Fzd7　could　abrogate　the　inhibitory　effect　of　miR-613　on　cell　proliferation　and　invasion　as　well　as　Wnt　signaling.　Additionally,　these　results　were　further　strengthened　by　data　showing　that　miR-613　was　significantly　downregulated　in　prostate　cancer　tissues,　exhibiting　an　inverse　correlation　with　Fzd7　expression.　In　conclusion,　our　study　suggests　that　miR-613　functions　as　a　tumor　suppressor,　partially　through　targeting　Fzd7,　and　is　a　potential　therapeutic　target　for　prostate　cancer.　(C)　2015　Elsevier　Inc.　All　rights　reserved.