The　remarkable　mechanical　and　electronic　properties　of　carbon　nanotubes　(CNTs)　make　them　ideal　materials　candidates　for　several　energy　and　structural　applications.　Understanding　the　interfacial　stress　transferred　from　CNTs　to　polymer　matrix　in　the　composite　is　crucial　for　exploring　CNTs　future　applications.　In　this　report,　a　reliable　method　was　developed　to　quantitatively　measure　the　interface　energy　between　nitrogen　doped　CNTs　and　poly(methyl　methacrylate)　(PMMA)　matrix.　By　pushing　the　PMMA　pillar　into　CNTs　arrays　protruded　on　the　substrate　then　pulling　it　out,　the　maximum　separation　force　and　adhesion　energy　were　detected.　With　finite　element　simulation　and　analysis　formula,　the　interface　energy　between　CNTs　and　PMMA　was　calculated.　The　results　showed　that　the　interface　energy　between　nitrogen　doped　multi-wall　CNTs　and　PMMA　was　obtained　at　54　Mpa　and　99　Mpa,　which　was　higher　than　pulling　pristine　multi-wall　CNTs　or　single-wall　CNTs　out　from　polymer　matrix.