In　recent　years,　microbial　colonization　on　the　surface　of　biomedical　implants/devices　has　become　a　severe　threat　to　human　health.　Herein,　surface　immobilized　guanidine　derivative　block　copolymers　create　an　antimicrobial　and　antifouling　dual-functional　coating.　We　report　the　preparation　of　an　antimicrobial　and　antifouling　block　copolymer　by　the　conjugation　of　polyhexanide　(PHMB)　with　either　ally　glycidyl　ether　or　allyloxy　polyethylene　glycol　(APEG;　MW　1200　and　2400):　The　allyl　glycidyl　ether　modified　PHMB　(A-PHMB)　and　allyloxy　polyethylene　glycol(1200/2400)　modified　PHMB　(APEG(1200/2400)-PHMB)　copolymers　were　grafted　onto　a　silicone　rubber　surface　as　a　bottlebrush-like　coating,　respectively,　using　a　plasma-UV-assisted　surface-initiated　polymerization.　Both　A-PHMB　and　APEG(1200/2400)-PHMB　coatings　exhibited　excellent　broad-spectrum　antimicrobial　properties　against　Gram-negative/positive　bacteria　and　fungi.　The　APEG(2400)-PHMB　coating　displayed　an　improved　antibiofilrn　as　well　as　antifouling　properties　and　a　long　reusable　cycle,　compared　with　two　other　coatings,　due　to　its　abundant　PEG　blocks　among　those　copolymers.　Also,　the　APEG(2400)-PHMB-coated　silicone　coupons　were　biocompatible　toward　mammalian　cells,　as　revealed　by　in　vitro　hemocompatibile　and　cytotoxic　assays.　An　in　vivo　study　showed　a　significant　decline　of　Escherichia　coli　colonies　with　a　5-log　reduction,　indicating　the　APEG(2400)-PHMB　coating　surface　worked　effectively　in　the　rodent　subcutaneous　infection　model.　This　PHMB-based　block　copolymer　coating　is　believed　to　be　an　effective　strategy　to　prevent　biomaterial-associated　infections.