Continually　phytochemical　study　of　the　roots　of　Heracleum　dissectum　had　led　to　the　isolation　of　three　previously　undescribed　polyacetylene　glycosides　(1-3),　together　with　seven　known　compounds,　including　one　polyacetylene　(8)　and　six　coumarins　(4-7　and　9-10)　using　diverse　chromatographic　methods.　The　structures　of　these　three　new　compounds　were　characterized　and　identified　as　4,　6-decadiyne-1-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside　(1),　(8Z)-decaene-4,　6-diyn-1-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside　(2)　and　(8E)-decaene-4,　6-diyn-1-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside　(3)　on　the　basis　of　their　physicochemical　properties　and　extensive　analyses　of　various　spectroscopic　data.　Their　triglycerides　accumulating　activities　were　assayed　and　the　results　showed　that　three　new　polyacetylene　glycoside　(1-3)　exhibited　triglycerides　accumulating　activities　in　3T3-L1　adipocytes.