The　fibrocartilaginous　enthesis　regeneration　after　rotator　repair　is　still　a　major　challenge.　Although　magnesium-based　alloy　orthopedic　implant　is　effective　to　promote　fibrocartilage　formation　at　the　tendon-bone　interface　in　the　anterior　cruciate　ligament　reconstruction　model,　it　was　limited　in　the　rotator　cuff　repair　for　its　special　anatomical　structure.　Herein,　we　developed　a　multifunctional　self-healing　magnesium　ions-quaternized　chitosan/Pluronic®　F127　(Mg-QCS/PF)　hydrogels　to　achieve　in-situ　and　customized　release　of　Mg2+,　and　demonstrated　the　sustained　release　of　Mg2+　from　the　hydrogel　to　significantly　promote　the　rotator　cuff　repair　in　the　rabbit　rotator　cuff　tear　model.　The　obtained　hydrogels　showed　excellent　self-healing　and　anti-compressive　performance.　Additionally,　the　good　injectability　and　adhesive　properties　of　the　hydrogels　make　it　easier　and　stable　to　deliver　Mg2+　at　the　tendon-bone　interface　with　irregular　shapes.　The　release　of　Mg2+　from　Mg-QCS/PF　hydrogels　improved　the　adhesion,　proliferation　and　migration　of　bone　mesenchymal　stem　cells　(BMSCs)　and　MC3T3　cells　in　vitro　compared　with　QCS/PF　without　Mg2+.　Furthermore,　the　composite　hydrogels　significantly　enhanced　the　fibrocartilaginous　interface　regeneration　in　the　rabbit　rotator　cuff　tear　model　in　terms　of　repaired　tendon　mature　scores,　fibrocartilage　regeneration,　collagen　remodeling　and　biomechanical　properties.　This　is　the　first　study　to　demonstrate　the　positive　effects　of　Mg2+　for　the　rotator　cuff　healing　in　the　rabbit　preclinical　model,　and　the　results　indicate　that　the　acellular　injectable　self-healing　Mg-doped　hydrogels　are　candidates　to　effectively　promote　in　situ　regeneration　of　rotator　cuff.　©　2020　Elsevier　B.V.