©　2017,　Editorial　Board　of　Journal　of　Xi＇an　Jiaotong　University　(Medical　Sciences).　All　right　reserved.　Objective:　To　produce　the　biological　calcium　citrate　cement　with　fresh　oyster　shells,　and　investigate　its　compressive　strength　and　biocompatibility　so　as　to　provide　the　experimental　basis　for　clinical　application　of　the　material.　Methods:　The　compressive　strength　of　biological　calcium　citrate　cement　was　measured　and　its　surface　morphology　was　observed　by　SEM.　The　calcium　release　curve　and　pH　value　were　measured　in　the　simulated　body　fluid.　Last,　its　biocompatibility　was　detected　by　cytotoxicity　test.　Results:　Biological　calcium　citrate　cement　produced　by　0.33　mL/g　liquid　solid　ratio　had　the　maximum　compressive　strength,　and　the　crystal　structure　of　the　material　was　uniform　and　orderly.　The　determination　of　pH　value　showed　that　the　degradation　and　absorption　of　biological　calcium　citrate　cement　did　not　significantly　change　the　pH　value　of　the　body　fluid.　With　gradual　degradation　of　the　material,　the　concentration　of　Ca2+　in　the　solution　increased　gradually.　Cytotoxicity　test　showed　that　this　material　had　good　biocompatibility　and　no　cytotoxicity.　Conclusion:　Biological　calcium　citrate　cement　possesses　strong　compressive　strength　and　good　biocompatibility,　and　it　can　form　a　microenvironment　with　low　in　alkaline　and　high　in　calcium.