The　use　of　antimicrobial　materials,　for　example,　silver　nanoparticles,　has　been　a　cause　for　concern　because　they　often　exert　an　adverse　effect　on　environmental　and　safety　during　their　preparation　and　use.　In　this　study,　we　report　a　class　of　green　antimicrobial　coating　based　on　a　supramolecular　assembly　of　a　protein　extracted　from　daily　food,　without　the　addition　of　any　other　hazardous　agents.　It　is　found　that　a　self-assembled　nanofilm　by　mere　hen　egg　white　lysozyme　has　durable　in　vitro　and　in　vivo　broad-spectrum　antimicrobial　efficacy　against　Gram-positive/negative　and　fungi.　Such　enhanced　antimicrobial　capability　over　native　lysozyme　is　attributed　to　a　synergistic　combination　of　positive　charge　and　hydrophobic　amino　acid　residues　enriched　on　polymeric　aggregates　in　the　lysozyme　nanofilm.　Accompanied　with　high　antimicrobial　activity,　this　protein-based　PTL　material　simultaneously　exhibits　the　integration　of　multiple　functions　including　antifouling,　antibiofilm,　blood　compatibility,　and　low　cytotoxicity　due　to　the　existence　of　surface　hydration　effect.　Moreover,　the　bioinspired　adhesion　mediated　by　the　amyloid　structure　contained　in　the　nanofilm　induces　robust　transfer　and　self-adhesion　of　the　material　onto　virtually　arbitrary　substrates　by　a　simple　one-step　aqueous　coating　or　solvent-free　printing　in　1　min,　thereby　allowing　an　ultrafast　route　into　practical　implications　for　surface-functionalized　commodity　and　biomedical　devices.　Our　results　demonstrate　that　the　application　of　pure　proteinaceous　substance　may　afford　a　cost-effective　green　biomaterial　that　has　high　antimicrobial　activity　and　low　environmental　impact.