To　study　the　attachment,　proliferation　and　differenciation　of　neural　stem　cells　(NSCs)　on　surface　modified　PHBHHx　films　and　to　establish　the　theory　of　PHBHHx　application　in　NSCs-based　brain　tissue　engineering.　PHBHHx　film　was　fabricated　by　a　solution-casting　method,　and　the　morphology　of　the　film　was　observed　under　scanning　electron　microscopy　(SEM).　The　films　were　treated　by　NaOH　or　lipase,　then　the　surface　hydrophilic　property　was　characterized　using　water　contact　angle　measurement.　NSCs　were　isolated　from　the　cerebral　cortex　of　rat　embryos　on　embryonic　day　14.5,　and　cultured　on　surface　treated　PHBHHx　films.　The　morphology　of　NSCs　attached　on　the　film　was　visualized　under　SEM,　and　the　survival　and　differentiation　of　NSCs　were　observed　through　immunocytochemical　staining.　Compared　with　the　untreated　PHBHHx　films,　the　water　contact　angle　of　NaOH　or　lipase　treated　PHBHHx　films　decreased　dramatically,　and　the　number　of　NSCs　attached　significantly　increased.　NSCs　survived　well　on　treated　PHBHHx　films　and　differentiated　into　neurons　and　glial　cells.　The　amelioration　of　hydrophilic　property　of　PHBHHx　film　improved　its　biocompatibility　with　NSCs.　PHBHHx　can　serve　as　a　novel　CNS　tissue　engineering　biomaterial　applied　for　NSCs　transplantation,　brain　repairing　and　regeneration.　©　2012　Chin　J　Biotech,　All　rights　reserved.